1-Step qPCR Buffer, 4x is a novel buffer system that has been designed for highly reproducible, accurate reverse transcription followed by qPCR in a single tube under fast thermal cycling conditions, delivering excellent results in fast RT-qPCR assays.
1-Step qPCR Buffer is a combination of the latest advances in buffer chemistry together with enhancers and stabilisers. The final mix still requires the addition of dNTPs and MgCl2.
- Used to create ultra-sensitive RT-qPCR assays to deliver reproducible results from even very low copy number RNA targets
- Robust formulation delivers consistent, reliable one-step RT-PCR performance in fast cycling conditions
- Minimises non-specific amplification for improved assay sensitivity and reliability
- High efficiency, giving excellent performance in multiplex qPCR assays
This product has met the following criteria to qualify for the following awards: